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Fieldwork

Moorea 2022 Adult Coral Stressor Experiment

  • 45 min read

This post includes the full notebook for Sept-Dec 2022 Acropora pulchra adult coral experiment Moorea, French Polynesia. These notebook posts are updated in order of most recent to oldest.

Project Overview

The goal of this project is to expose adult Acropora pulchra corals to conditions that elicit changes in symbiotic and metabolic condition and test how these changes influence response to a repeat stressor. Here, we are manipulating light and feeding conditions to elicit metabolic and symbiotic responses and testing stress response at the end of the experiment using the thermal stress CBASS system. The team will expose adult corals to light with added food, light with no added food, and shade with added food in Sept - Dec with thermal stress phenotype measurement in Dec with S. Matsuda and R. Cunning.

The experimental design overview can be seen here:

e5design

This post details activities and notebooks for this project.

Field expedition members:
Ariana Huffmyer, URI
Pierrick Harnay, URI
Danielle Becker-Polinski, URI
Hollie Putnam, URI
Shayle Matsuda, Shedd Aquarium
Ross Cunning, Shedd Aquarium

Helpful links

The following protocols and links to data will be used regularly by the field team conducting this project.

Daily measurements:
Daily measurements protocol
Daily measurement data sheet
Schedule daily checklist

Weekly maintenance:
Weekly maintenance protocol
Schedule weekly checklist

Fragment metadata and sample tracking:
Fragment tracking datasheet
Sample inventory

Baseline sampling:
Baseline sampling protocol
Baseline sampling checklist
Sample inventory

Logger files:
Logger files folder

Feeding protocol:
Feeding protocol

Read More

14 March 22 - Gametogenesis and Heatwave Research

  • 1 min read

Tank Updates

Danielle and MVP Gump employee, Tony, have been working on getting a chiller system set-up for the water tables at Gump and today was the day! Using Hollies idea for a vanne à flotteur system where the water can be cooled and used but will always stay above a certain level to not damage the pumps or the chiller. This set-up will be a great addition to control ambient temperature levels for the planned tank system.

Read More

13 March 22 - Gametogenesis and Heatwave Research

  • ~1 min read

Tank Updates

Danielle assigned the remaining 6 Apex temperature probes to their respective Apex PM1 units to prep them for setting up during the heatwave. She also made all the settings and outlet updates for the second Apex Controller System to follow her planned ramping of temperatures. All of the outlets are linked to the Putnam Lab Apex fusion account to be able to set planned diel temperatures each day. Danielle also began breaking down the 6 large tanks she had set-up for preliminery testing.

Read More

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Sampling

Moorea 2022 Adult Coral Stressor Experiment

  • 45 min read

This post includes the full notebook for Sept-Dec 2022 Acropora pulchra adult coral experiment Moorea, French Polynesia. These notebook posts are updated in order of most recent to oldest.

Project Overview

The goal of this project is to expose adult Acropora pulchra corals to conditions that elicit changes in symbiotic and metabolic condition and test how these changes influence response to a repeat stressor. Here, we are manipulating light and feeding conditions to elicit metabolic and symbiotic responses and testing stress response at the end of the experiment using the thermal stress CBASS system. The team will expose adult corals to light with added food, light with no added food, and shade with added food in Sept - Dec with thermal stress phenotype measurement in Dec with S. Matsuda and R. Cunning.

The experimental design overview can be seen here:

e5design

This post details activities and notebooks for this project.

Field expedition members:
Ariana Huffmyer, URI
Pierrick Harnay, URI
Danielle Becker-Polinski, URI
Hollie Putnam, URI
Shayle Matsuda, Shedd Aquarium
Ross Cunning, Shedd Aquarium

Helpful links

The following protocols and links to data will be used regularly by the field team conducting this project.

Daily measurements:
Daily measurements protocol
Daily measurement data sheet
Schedule daily checklist

Weekly maintenance:
Weekly maintenance protocol
Schedule weekly checklist

Fragment metadata and sample tracking:
Fragment tracking datasheet
Sample inventory

Baseline sampling:
Baseline sampling protocol
Baseline sampling checklist
Sample inventory

Logger files:
Logger files folder

Feeding protocol:
Feeding protocol

Read More

14 March 22 - Gametogenesis and Heatwave Research

  • 1 min read

Tank Updates

Danielle and MVP Gump employee, Tony, have been working on getting a chiller system set-up for the water tables at Gump and today was the day! Using Hollies idea for a vanne à flotteur system where the water can be cooled and used but will always stay above a certain level to not damage the pumps or the chiller. This set-up will be a great addition to control ambient temperature levels for the planned tank system.

Read More

13 March 22 - Gametogenesis and Heatwave Research

  • ~1 min read

Tank Updates

Danielle assigned the remaining 6 Apex temperature probes to their respective Apex PM1 units to prep them for setting up during the heatwave. She also made all the settings and outlet updates for the second Apex Controller System to follow her planned ramping of temperatures. All of the outlets are linked to the Putnam Lab Apex fusion account to be able to set planned diel temperatures each day. Danielle also began breaking down the 6 large tanks she had set-up for preliminery testing.

Read More

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Gametogenesis

14 March 22 - Gametogenesis and Heatwave Research

  • 1 min read

Tank Updates

Danielle and MVP Gump employee, Tony, have been working on getting a chiller system set-up for the water tables at Gump and today was the day! Using Hollies idea for a vanne à flotteur system where the water can be cooled and used but will always stay above a certain level to not damage the pumps or the chiller. This set-up will be a great addition to control ambient temperature levels for the planned tank system.

Read More

13 March 22 - Gametogenesis and Heatwave Research

  • ~1 min read

Tank Updates

Danielle assigned the remaining 6 Apex temperature probes to their respective Apex PM1 units to prep them for setting up during the heatwave. She also made all the settings and outlet updates for the second Apex Controller System to follow her planned ramping of temperatures. All of the outlets are linked to the Putnam Lab Apex fusion account to be able to set planned diel temperatures each day. Danielle also began breaking down the 6 large tanks she had set-up for preliminery testing.

Read More

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ExperimentalDesign

14 March 22 - Gametogenesis and Heatwave Research

  • 1 min read

Tank Updates

Danielle and MVP Gump employee, Tony, have been working on getting a chiller system set-up for the water tables at Gump and today was the day! Using Hollies idea for a vanne à flotteur system where the water can be cooled and used but will always stay above a certain level to not damage the pumps or the chiller. This set-up will be a great addition to control ambient temperature levels for the planned tank system.

Read More

13 March 22 - Gametogenesis and Heatwave Research

  • ~1 min read

Tank Updates

Danielle assigned the remaining 6 Apex temperature probes to their respective Apex PM1 units to prep them for setting up during the heatwave. She also made all the settings and outlet updates for the second Apex Controller System to follow her planned ramping of temperatures. All of the outlets are linked to the Putnam Lab Apex fusion account to be able to set planned diel temperatures each day. Danielle also began breaking down the 6 large tanks she had set-up for preliminery testing.

Read More

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Heatwave

14 March 22 - Gametogenesis and Heatwave Research

  • 1 min read

Tank Updates

Danielle and MVP Gump employee, Tony, have been working on getting a chiller system set-up for the water tables at Gump and today was the day! Using Hollies idea for a vanne à flotteur system where the water can be cooled and used but will always stay above a certain level to not damage the pumps or the chiller. This set-up will be a great addition to control ambient temperature levels for the planned tank system.

Read More

13 March 22 - Gametogenesis and Heatwave Research

  • ~1 min read

Tank Updates

Danielle assigned the remaining 6 Apex temperature probes to their respective Apex PM1 units to prep them for setting up during the heatwave. She also made all the settings and outlet updates for the second Apex Controller System to follow her planned ramping of temperatures. All of the outlets are linked to the Putnam Lab Apex fusion account to be able to set planned diel temperatures each day. Danielle also began breaking down the 6 large tanks she had set-up for preliminery testing.

Read More

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Physiology

Moorea 2022 Adult Coral Stressor Experiment

  • 45 min read

This post includes the full notebook for Sept-Dec 2022 Acropora pulchra adult coral experiment Moorea, French Polynesia. These notebook posts are updated in order of most recent to oldest.

Project Overview

The goal of this project is to expose adult Acropora pulchra corals to conditions that elicit changes in symbiotic and metabolic condition and test how these changes influence response to a repeat stressor. Here, we are manipulating light and feeding conditions to elicit metabolic and symbiotic responses and testing stress response at the end of the experiment using the thermal stress CBASS system. The team will expose adult corals to light with added food, light with no added food, and shade with added food in Sept - Dec with thermal stress phenotype measurement in Dec with S. Matsuda and R. Cunning.

The experimental design overview can be seen here:

e5design

This post details activities and notebooks for this project.

Field expedition members:
Ariana Huffmyer, URI
Pierrick Harnay, URI
Danielle Becker-Polinski, URI
Hollie Putnam, URI
Shayle Matsuda, Shedd Aquarium
Ross Cunning, Shedd Aquarium

Helpful links

The following protocols and links to data will be used regularly by the field team conducting this project.

Daily measurements:
Daily measurements protocol
Daily measurement data sheet
Schedule daily checklist

Weekly maintenance:
Weekly maintenance protocol
Schedule weekly checklist

Fragment metadata and sample tracking:
Fragment tracking datasheet
Sample inventory

Baseline sampling:
Baseline sampling protocol
Baseline sampling checklist
Sample inventory

Logger files:
Logger files folder

Feeding protocol:
Feeding protocol

Read More

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Respirometry

14 March 22 - Gametogenesis and Heatwave Research

  • 1 min read

Tank Updates

Danielle and MVP Gump employee, Tony, have been working on getting a chiller system set-up for the water tables at Gump and today was the day! Using Hollies idea for a vanne à flotteur system where the water can be cooled and used but will always stay above a certain level to not damage the pumps or the chiller. This set-up will be a great addition to control ambient temperature levels for the planned tank system.

Read More

13 March 22 - Gametogenesis and Heatwave Research

  • ~1 min read

Tank Updates

Danielle assigned the remaining 6 Apex temperature probes to their respective Apex PM1 units to prep them for setting up during the heatwave. She also made all the settings and outlet updates for the second Apex Controller System to follow her planned ramping of temperatures. All of the outlets are linked to the Putnam Lab Apex fusion account to be able to set planned diel temperatures each day. Danielle also began breaking down the 6 large tanks she had set-up for preliminery testing.

Read More

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Spawning

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Hawaii

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twitter

News

  • 1 min read

Thinking Big: Research team awarded NSF grant to discover “Rules of Life” through the study of coral reef ecosystems

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Moorea

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lab

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video

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wyze

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Reproduction

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Apulchra

Moorea 2022 Adult Coral Stressor Experiment

  • 45 min read

This post includes the full notebook for Sept-Dec 2022 Acropora pulchra adult coral experiment Moorea, French Polynesia. These notebook posts are updated in order of most recent to oldest.

Project Overview

The goal of this project is to expose adult Acropora pulchra corals to conditions that elicit changes in symbiotic and metabolic condition and test how these changes influence response to a repeat stressor. Here, we are manipulating light and feeding conditions to elicit metabolic and symbiotic responses and testing stress response at the end of the experiment using the thermal stress CBASS system. The team will expose adult corals to light with added food, light with no added food, and shade with added food in Sept - Dec with thermal stress phenotype measurement in Dec with S. Matsuda and R. Cunning.

The experimental design overview can be seen here:

e5design

This post details activities and notebooks for this project.

Field expedition members:
Ariana Huffmyer, URI
Pierrick Harnay, URI
Danielle Becker-Polinski, URI
Hollie Putnam, URI
Shayle Matsuda, Shedd Aquarium
Ross Cunning, Shedd Aquarium

Helpful links

The following protocols and links to data will be used regularly by the field team conducting this project.

Daily measurements:
Daily measurements protocol
Daily measurement data sheet
Schedule daily checklist

Weekly maintenance:
Weekly maintenance protocol
Schedule weekly checklist

Fragment metadata and sample tracking:
Fragment tracking datasheet
Sample inventory

Baseline sampling:
Baseline sampling protocol
Baseline sampling checklist
Sample inventory

Logger files:
Logger files folder

Feeding protocol:
Feeding protocol

Read More

Back to Top ↑

Light

Moorea 2022 Adult Coral Stressor Experiment

  • 45 min read

This post includes the full notebook for Sept-Dec 2022 Acropora pulchra adult coral experiment Moorea, French Polynesia. These notebook posts are updated in order of most recent to oldest.

Project Overview

The goal of this project is to expose adult Acropora pulchra corals to conditions that elicit changes in symbiotic and metabolic condition and test how these changes influence response to a repeat stressor. Here, we are manipulating light and feeding conditions to elicit metabolic and symbiotic responses and testing stress response at the end of the experiment using the thermal stress CBASS system. The team will expose adult corals to light with added food, light with no added food, and shade with added food in Sept - Dec with thermal stress phenotype measurement in Dec with S. Matsuda and R. Cunning.

The experimental design overview can be seen here:

e5design

This post details activities and notebooks for this project.

Field expedition members:
Ariana Huffmyer, URI
Pierrick Harnay, URI
Danielle Becker-Polinski, URI
Hollie Putnam, URI
Shayle Matsuda, Shedd Aquarium
Ross Cunning, Shedd Aquarium

Helpful links

The following protocols and links to data will be used regularly by the field team conducting this project.

Daily measurements:
Daily measurements protocol
Daily measurement data sheet
Schedule daily checklist

Weekly maintenance:
Weekly maintenance protocol
Schedule weekly checklist

Fragment metadata and sample tracking:
Fragment tracking datasheet
Sample inventory

Baseline sampling:
Baseline sampling protocol
Baseline sampling checklist
Sample inventory

Logger files:
Logger files folder

Feeding protocol:
Feeding protocol

Read More

Back to Top ↑