ITS2 Data

Internal Transcribed Spacer 2 sequences and analysis

Interactive ITS2 Data Explorer

This page provides interactive visualization and searchable tables for Internal Transcribed Spacer 2 (ITS2) relative abundance data across coral species and time points.

ITS2 sequences are important molecular markers used for coral symbiont identification and community analysis, particularly useful for studying changes in symbiont communities over time.

Data Overview

**Dataset Summary:**
- Total samples: 441 
- Time points: 4 
- Species: 3 
- Sites: 3 
- ITS2 types: 40
ITS2 Data Summary by Species and Time Point
Species Time Point Sample Count Sites
Acropora timepoint1 42 Manava, Mahana, Hilton
Acropora timepoint2 23 Manava, Mahana, Hilton
Acropora timepoint3 21 Manava, Mahana, Hilton
Acropora timepoint4 34 Manava, Mahana, Hilton
Pocillopora timepoint1 40 Mahana, Hilton, Manava
Pocillopora timepoint2 42 Mahana, Hilton, Manava
Pocillopora timepoint3 39 Mahana, Hilton, Manava
Pocillopora timepoint4 38 Mahana, Hilton, Manava
Porites timepoint1 44 Mahana, Hilton, Manava
Porites timepoint2 42 Mahana, Hilton, Manava
Porites timepoint3 40 Mahana, Hilton, Manava
Porites timepoint4 36 Mahana, Hilton, Manava

Time Series Visualization

Interactive Data Tables

Download ITS2 Data

Data source: ITS2_rel_abund_matrix.csv

Interactive Data Table

Data Sources and Methods

ITS2 Sequencing

Internal Transcribed Spacer 2 (ITS2) is a highly variable DNA region located between the 5.8S and 28S ribosomal RNA genes. This region is commonly used for:

  • Coral symbiont identification
  • Symbiodiniaceae community analysis
  • Temporal changes in symbiont communities
  • Response to environmental stressors

Data Structure

This dataset contains relative abundance values for different ITS2 types across:

  • Timepoints: 4 time points (timepoint1-4) representing temporal sampling
  • Sites: Multiple reef sites with different environmental conditions
  • Nutrient Treatments: High and low nutrient conditions
  • Species: Multiple coral species including Acropora and others
  • ITS2 Types: Relative abundance values for ~40 different ITS2 sequence variants

Data Processing

  1. Sequence Quality Control: Raw ITS2 sequences underwent quality filtering
  2. Taxonomic Assignment: Sequences were assigned to ITS2 type profiles using SymPortal
  3. Relative Abundance: Count data was normalized to relative abundance within each sample
  4. Matrix Format: Data organized with samples as rows and ITS2 types as columns

Download

The complete dataset is available for download using the button provided above. The file contains: - Sample metadata (colony ID, timepoint, site, nutrient treatment, species) - Relative abundance values for all detected ITS2 types